Generator

Part:BBa_I754001:Design

Designed by: Mahinur Akkaya   Group: iGEM07_Turkey   (2007-06-27)


ech protein generator for vanillin biosynthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 892
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 892
    Illegal NotI site found at 836
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 892
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 892
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

needs to be used in conjuction with fcs in order to generate vanillin.


Source

uses "partinfo" BBa_l75400 as well as a promoter, RBS, and terminator. IPTG induceable promoter inordder to test the conctruct. Strong RBS and terminator.

References